Pier Carlo Montecucchi

Certified Professioal Consultant

Chartered Consultant

Industry sector: Biotechnology - Proteomics

Expert at the Court in Turin (Italy) - Sector: Biology

Via delle Rosine n. 1 - 10123 Torino (I)

540 Beverly Court,  Suite 1 - Tallahassee FL 32301  (.U.S.A); phone: 850-561-0604

mobile: (011-39) 340-544-1926 (from U.S.A.)

Public key for cryptography :

The PGP Certificate Server. (MIT PGP Public Key Server) http://pgp.mit.edu

Descendant Chart for Pier Carlo Montecucchi

PERSONAL SUMMARY

Professional with other twenty years' experience in peptide / protein chemistry with a strong research background. Experienced in new product development, core chemistry research and engineering, product management, marketing / communications. Working and consulting with biotech companies. Excellent leadership, problem solving and decision making capabilities related to the manufacturing area, research lab and board room. Skilled in managing and motivating highly technical professionals. Highly organized and decisive with the proven ability to maintain multiple priorities in business, technology and research. Strong theoretical background in Bio-Organic Chemistry.

Professional Affiliations

• Professional Register of Italian Biologists (Ordine Nazionale dei Biologi)
• DNA Repair Interest Group ( web site:http://www.nih.gov:80/sigs/dna-rep/ )
• Human Proteome Organization [Italian section (web site: http://www.hupo.it )]
• Italian Society for Biochemistry and Molecular Biology
• The Biometric Consortium

Academic Credentials

• "Liceo Classico Lorenzo Costa" in La Spezia (SP, Italy)i
• University of Turin (Italy) - "summa cum laude" Doctorate in Biology
• Scuola dìApplicazione d'Arma: Elementi di nautica per la condotta di imbarcazioni da diporto (under baval captain Mario De Petris), Turin (Italy), 1963
• Naval Military Academy (Livorno, Italy) and the Navy Arsenal (MARICOMMI, La Spezia, Italy), as Lieutenant
• Ministry of Public Education (Italy) :  Professor of chemistry, biology, and geography in the high schools ( O.M. dated 10/8/1967 Gazzetta Ufficiale 202 dated 12/8/1967 , Class XV))
• Assistant lecturer at the University of Turin, Italy  (Department of Biological Chemistry, Faculty of Medicine)
• C.E.S.A.M. - Cour de Methodologie M1 - Villejuif (France) (Carte d'immatriculation 409) (1970 - 1971)
• Bocconi University in Milano (Italy) - Diploma of the Executive Course in Business Administration
• The Consultants Institute in Princess Anne (MD, USA) - Certified Professional Consultant ; Chartered Consultant
• A diver's brevet issued by F.I.P.S. (now F.I.P.S.A.S.) (brevet n. 151 – Feb.6, 1965)
• Graduate seminars in peptide chemistry at the Politecnico Milan (Italy) ("Scuola di Specializzazione in Sintesi Organica" dedicated to A. Quilico)
• Conferences and Research Seminars to international symposia and NATO specialization schools
• Ad Hoc Reviewer for international scientific magazines
• Member of the "Associazione Nazionale Carabinieri - Sez. Torino (Italy)"
• Expert at the Court in Turin (Italy) - Sector: Biology (Perito & Consulente Tecnico presso il Tribunale di Torino Italia - Categoria: Biologi)

Giuro di essere fedele alla Repubblica Italiana, di osservarne la Costituzione e le Leggi e di adempiere con disciplina ed onore tutti i doveri del mio Stato per la difesa della Patria e la salvaguardia delle libere istituzioni.

Lo giurate Voi?.

Lo giuro!.

Honors

• Post Doctoral Fellowship from the CNR (National Council of Research)  (Torino, Italy) (1966)
• Post Doctoral Fellowship from CNEN (National Committee for Nuclear Energy) (CNEN - EURATOM Fiascherino, SP, Italy) (1967)
• Visiting Scientist at the Genetics Department - University of Pavia (Italy) (1972)
• Visiting Scientist at The Pharmacology Department - University of Rome (Italy) (1974)
• Visiting Scientist at The Max Planck Institute for Biochemistry (Martinsried b. Munchen) (1978)
• Meyerhoff Professorship at The Weizmann Institute of Science (Rehovot - Israel) (1982 - 1983)
• Scientist at The Montedison Group (Milano, Italy) (1983)
• Visiting Scientist at the Istitute of Cellular Biology - CNR  (National Council of Research), Rome Pavia (Italy) (1986)
• Judge at the Capitol Regional Science and Engineering Fair 
• (Florida State University, Tallahassee, FL, U.S.A.) (1992)
• Gold Medal: 1967 - 2007 : Forty years "Ordine Nazionale dei Biologi"

Certifications

Certified Professional Consultant ; Chartered Consultant  [The Consultants Institute in Princess Anne (MD, USA)]

Fellowships

• 1982 - 1984  Meyerhoff professorship - The Weizmann Institute of Science - Genetics Department - Rehovot (Israel), under prof. L. Sachs
• 1967 - 1968  Postdoctoral fellowship CNEN at the CNEN - EURATOM - Laboratory for the Study of Marine Radioactiviy - Fiascherino (SP, Italy), under dr. M. Bernhard
• 1966 - 1967  Postdoctoral fellowship CNR - Department of Biochemistry - School of Medicine - University of Turin (Italy), under prof. C. Lenti
• 1965 - 1968 Assistant - Department of Biological Chemistry - School of Medicine - University of Turin (Italy), under prof. C. Lenti

Stages

• 1990 - 1992 - The Florida State University - Department of Biological Sciences - Tallahassee (FL, U.S.A.), under prof. M. Freeman (Object of research: :isolation of new hypothalamic peptides)
• 1986 -Institute of Cell Biology - CNR (Council National Research) Rome (Italy) under prof. R. Revoltella (Object of research: differentiation proteins)
• 1982 - 1983 The Weizmann Institute of Science - Genetics Department - Rehovot (Israel), under prof. L. Sachs (Object of research: differentiation proteins)
• 1978 - 1979 - Max Planck Institute for Biochemistry (Martinsried, Germany) - Department  of  Protein Chemistry  under prof. G. Braunitzer and prof.  A. Henschen Edman (Object of research: protein structure determination)
• 1974  - University of Rome (Italy - Institute of Pharmacology under prof. V. Erspamer (Object of research: identification of new peptides from the extracts of amphibian skins)
• 1972  - University of Pavia (Italy) - Institute of Genetics  under prof. O.Ciferri (Object of research: isolation of ds RNA as interferon's inducer)

Areas of Expertise

• Analytical methods development (electrophoresis, 2d gel analysis, HPLC, TLC, spectrophotometric and fluorimetric analyses)
• Enzymatic catalysis
• Immobilization of enzymes (solid / liquid support)
• Nucleic acid extraction and purification (chromatography, centrifugation, electrophoresis)
• Peptide synthesis (solid phase synthesis, operation management for a bulk peptide manufacturing facility)
• Electrophysiology (determination of mammalian ventricular potential action: membrane potential, overshoot)
• Process development [purification of proteins on large scale, operation management for a bulk peptide manufacturing facility, silk production (selection of Bombyx mori cocoons)]
• Purification of peptides / proteins (affinity chromatography, centrifugation, counter current distribution, displacement chromatography, 2d gel analysis, electrophoresis, filtration, HPLC, FPLC, liquid chromatography, microbore HPLC, paper chromatography, partition chromatography, TLC, HVPE)
• Peptide / protein structure determination [automatic / manual Edman degradation, ABI sequencer improvements, amino acid analyses (with derivatization techniques: FMOC, PITC, OPA, AccQ-Fluor reagent), chemical and enzymatic methods, circular dichroism, mass spectrometry analysis, PTH-amino acids identification, separation of fragments by HVPE, HPLC, TLC, identification of modifications due to post-translational processes]
• Analysis of  synthetic peptides and recombinant proteins to identify chemical degradation (deamination, isomerization, oxidation, proteolysis, disulfide exchange) or physical degradation (aggregation, unfolding, precipitation, adsorption)
• Pattern discovery from the amino acid sequences
• Process simulation and optimization via CAPD

Other Expertise

• Business plan preparation
• Intelligence analysis tools
• Licensing (in-, out-)(Commercialization of technologies under appointment by the  Court (Section: bankruptcy) in Milan (Italy)]
• Logistics (at Navy Arsenal - MARICOMMI, La Spezia, Italy)
• Marketing research
• NIH / ADAMHA Peer Review Consultant
• PC software (Windows 95, 98,  and NT v. 4.0, Microsoft Word, Excel and PowerPoint, Harvard Graphics, Harvard Project Management, Corel, SuperPro Designer, Internet Explorer, Outlook Express, crypto with PGP (Pretty Good Privacy), web page preparation (Microsoft FrontPage, NetObjects Fusion)]
• Securing e-business perimeter ["Safe Harbor" (U.S. Department of Commerce - export Portal at: http://www.export.gov/safeharbor/ )]; Biology and security
• Technology scouting
• Underwater activities with a diver's brevet issued by F.I.P.S. (now F.I.P.S.A.S.) (brevet n. 151 – Feb.6, 1965)

Career Accomplishments / Highlights

• Conducted scientific research at the University of Turin (Institute of Biological Chemistry) (Italy), University of Pavia (Genetics Institute) (Italy), University of Rome (Institute of Pharmacology) (Italy), CNEN-Euratom in Fiascherino (SP, Italy), at the Centro Applicazioni Militari Energia Nucleare in S.Pietro a Grado (PI, Italy), Montedison Group in Milano (Italy), Max Planck Institute for Biochemistry in Martinsried (Germany), The Weizmann Institute of Science in Rehovot (Israel) and at Florida State University in Tallahassee (FL, USA). Incharged for graduate seminars in peptide chemistry at the Politecnico Milano (Italy) ("Scuola di specializzazione in Sintesi Organica dedicated To A. Quilico).
• Discovered new peptides including bombesin-like peptides, dermorphins, tryptophyllins, sauvagines, hematopoietic differentiation factors, immunotoxins, new-low-molecular-weight immunoregulins.
• Identified enzymatic metabolic pathways, developed immobilized enzymes, low-molecular-weight supersulfated heparin, hemoglobin derivatives as blood substitutes and peptides for therapeutic applications.
• Identified partners for products' development.
• Offered intelligence outsource services to private and public organizations.

SCIENTIFIC RELEVANCE: Isolation and structure determination of new peptides from frog skin extracts (kassinin, litorin-2, hylambatin, dermorphins, sauvagines, tryptophyllins), rat hypothalamus (LH-release inhibiting factor) and from human leukocytes (new immunomodulatory molecules); characterization of new ribosome inactivating proteins and of a DNA -binging protein involved in human leukemic cell differentiation; isolation, sequencing and cloning of a new beta galactosidase archaeobacterial gene; sequence analysis of a protamine from a marine worm ( Chaetopterus variopedatus ) and of a dimeric myoglobin from a marine gastropod ; preparation of hemoglobin-based blood substitutes; isolation of DS RNA; isolation and characterization of the components of a new cellulase. In particular, referring to the isolated peptides, it is important to report that kassinin and sauvagines have anticipated the discovery of their mammalian counterparts, substance K and corticotropin releasing hormone (confirming the so-called "gut-skin-brain peptide triangle); dermorphins are the first example of Vertebrata peptides containing a D-amino acid residue in their sequences.

PROFESSIONAL WORKSTORY

• 1999 - Founder and C.E.O. of MONTEGEN, A Bureau for Biotechnology - Italy (Milano) and U.S.A. (Tallahassee, FL)
• 1997 - Founder of INTELLIGENCE-NET OFFICE - U.S.A. (Tallahassee, FL)
• 1993 to 1998 Hi - Tech Industry Consultants - Italy (Milano) and U.S.A. (Tallahassee, FL)
• 1993 to 1994 - Consultant for Sclavo S.p.A. Gruppo Marcucci - Siena (Italy)
• 1993 - Founder and C.E.O. of Hi-Tech Industry Consultants - Italy (Milano) and U.S.A. (Tallahassee, FL)
• 1990 to 1992 - Florida State University (Tallahassee, FL) - Biological Science Department as Visiting Professor
• 1988 to 1990 - Sclavo - Du Pont in Milano (Italy), as Director, Purification Unit
• 1970 to 1987 - Farmitalia Carlo Erba (Montedison Group) in Milano (Italy)
• 1969 - Centro Applicazioni Militari Energia Nucleare (C.A.M.E.N.) - Radiopathology Department (C.A.M.E.N.) (S. Piero a Grado, PI, Italy)
• 1968 - Laboratory for the Study of the Radioactive Contamination of the Sea ; CNEN - EURATOM (Fiascherino, SP, Italy)
• 1965 to 1967 - Military service in the Italian Navy at the Naval Academy (33^ Course "L" for AUC - MARINACCAD, Livorno, Italy) and at the Navy Arsenal (MARICOMMI, La Spezia, Italy), as Lieutenant

Bibliography

Publications via PubMed (NIH National Library of Medicine)

Research Accomplishments

Publications on books, abstracts and proceedings of symposia

Trade Marks' Registration

Patents

My Genealogy